聚酰胺分离纯化茶黄素类物质研究

Study on Separation and Purification of Theaflavins by Polyamide

本实验利用聚酰胺分离茶黄素类,研究发现其最佳的分离条件为:上样量250mg/50ml聚酰胺,上样浓度20%,洗脱剂为甲醇:氯仿:丙酮:冰醋酸=3:5:8:0.5,等度洗脱,流速0.6BV/h。得到TF、TF-3'-G两个组分,含量分别为93%、85%。这是一种简单、有效的茶黄素类分离方法。

This study investigated purification of theaflavins by polyamide. The optimal separation conditions were the volume of theaflavins 250 mg/50 ml polyamid, the sovlent system including methanol, chloroform, acetone and glacial acetic acid with their respective ratio as 3：5：8：0.3, and speed of washout 0.6 BV/h. Two components of TF and TF-3＇-G were obtained, and their concentrations were 93% and 85% respectively. This is a simple and effective method for separation and purification of the theaflavins.