羊栖菜多糖的提取及纯化

Extraction and Purification of Sargassum fusiforme Polysaccharides

目的:分离纯化羊栖菜多糖。方法:采用水煮醇沉法、酸煮醇沉法及乙醇脱脂后水煮醇沉三种方法提取羊栖菜粗多糖。利用Sevag法结合胰酶消化去蛋白,SephadexG200分离纯化多糖。结果:三种方法提取获得粗多糖含量分别为:24.35%、23.87%和17.87%。分子筛层析分离得到两个在A620和A280重合的组分,其硫酸根的含量分别为8.95%和3.92%。结论:从羊栖菜粗多糖分离得到两个含有硫酸基的蛋白聚糖。

Objective： To study on the methods of extraction and purification polysacchande from Sargassumfusiforme. Methods： Comparing three methods of extraction coarse polysaccharide from Sargassumfusiforme by hot water, acid hot water and hot water after degreased by ethanol. Crude polysaccharides were preliminarily purified with Sevag deprotein, and further purified by Sephadex G200 chromatography. Results： The 24.35%, 23.87% and 17.87% of crude polysaccharides were obtained from Sargassumfusiforme by these three methods, respectively. After purified, two types of protein polysaccharides were obtained fromSargassumfusiforme. The contents of their SO4^2- were 8.95% and 3.92%, respectively. Conclusion： Two protein polysaccharides containing sulfate were obtained from Sargassumfusiforme.