铜绿微囊藻(Microcystic aeruginosa)胞外和胞内酸性多糖分离、纯化方法的比较及产物的鉴定

Comparison of Separation and Purification Methods between Intra-and Extra-cellular Acidic Polysaccharides from Microcystic aeruginosa and Identification of Their Products

铜绿微囊藻(Microcystic aeruginosa)属于蓝藻,是水华爆发的主体藻类。本实验在不同pH值和温度条件下分别提取其胞内和胞外多糖的粗制品,采用DEAE-纤维素离子交换柱层析,分离出其中含有醛酸基团的酸性组分,再用具分子筛效应的SephadexG-150和G-100进一步纯化出具HPLC单一对称峰的两个胞外酸性多糖组分和一个胞内酸性多糖组分。分别测定和分析三种酸性多糖的分子量、糖醛酸及单糖的组成。

Microcystic aeruginosa, a kind of blue-green alga, is the main alga in algae bloom. In this study extra- and intra-cellular crude polysaccharides were purified from M.aeruginosa by different temperature and pH. Their acidic components which had alduronic acid groups were purified by DE-52, and then acidic polysaccharides of the two groups from intra-ceUular and one group from extra--cellular were purified by Sephadex columns. Each of these three acidic polysaceharides presents a single HPLC peak in its respective chromatographs of DE-52 and Sephadex G-150 columns. These three acidic polysaccharides are different from each other in their molecular weights, alduronic acid contents and monosaccharides constitutents.