摘要
本实验利用ESI-MS/MS对反相高效液相色谱分离的具有血管紧张素I转换酶(ACE)抑制活性的珠蛋白小肽的结构进行鉴定。结果表明:此肽的序列为Val-Val-Tyr-Pro-Trp-Thr(VVYPWT),位于猪的血红蛋白β链的34-39氨基酸序列片断,它对ACE有很好的抑制活性,其IC50为6.02μmol/L。
This paper described isolation and identification of angiotensin I-converting enzyme (ACE) inhibitory peptide derived from the peptic globin hydrolysate. After the isolation of ACE inhibitory peptide with reversed-phase high-performance liquid chromatography (RP-HPLC) on Cls column, one active fraction was obtained, The amino acid sequence was identified by electrospray ionization tandem mass spectrometry (ESI-MS-MS). The results showed that this peptide is ValVaI-Tyr-Pro-Trp-Thr(VVYPWT), corresponding to the 34-39 fragment of the β chain of porcine hemoglobin, with IC50 value as 6.02 μmol/L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第12期248-250,共3页
Food Science
基金
辽宁省自然科学基金项目(20052162)