碱性蛋白酶对黑麦草叶蛋白水解作用的研究(英文)

Technological Optimization for Alcalase Hydrolysis of Ryegrass Leaf Protein Concentrate (LPC)

通过单因素和 L9(34)正交试验,系统研究了碱性蛋白酶水解黑麦草叶蛋白的水解最佳工艺。确定出该酶水解黑麦草叶蛋白的最佳工艺参数是温度为 45℃,pH10.5,E/S=1750U/g,反应时间为 10h。利用高效液相色谱和氨基酸自动分析仪分析得出最佳工艺条件下的水解液中共有7个部分的肽段,其相对分子质量分布在102～14502之间,其中数均相对分子质量 249 的肽含量最多,约占 21.13%。氨基酸自动分析仪图谱分析得出最佳工艺条件下的酶解液中总游离氨基酸的含量为 3.233%,其中必需氨基酸占总氨基酸的 30.776%,鲜味氨基酸占 38.324%,药效氨基酸占 75.286%。

The effects ofpH, temperature and proportion of enzyme to substrate （E/S） on the hydrolysis of ryegrass leaf protein concentrate（LPC） with alcalase were investigated by single factors and L9（34） orthogonal test. The results indicated that the optimal pH, temperature and E/S for hydrolysis of ryegrass LPC were 10.5, 45 ~C and 1750 units per gram respectively. The protein hydrolysate analysis was performed by high performance liquid chromatography （HPLC）. There were 7 kinds of peptides. Their relative molecular weights are between 102 and 14502 and most of them are 249, accounted for 21.13 %. Amino acids analysis was performed by automatic amino acid analyzer （AAAA）. The total content of free amino acids in hydrolysates accounted for 3.233%. The proportion of essential amino acids to total free amino acids is 30.776%, while the proportion of sapor amino acids to total free amino acids is 38.324%, and the proportion of drug-effective amino acids to total free amino acids is 75.286%.