海藻酸钠包埋法固定化绿色木霉的研究

Study on Trichoderma viride Immobilization in Sodium Alginate

本实验以海藻酸钠包埋法固定化绿色木霉,研究了海藻酸钠固定化的最佳条件和固定化细胞理化性质。3%海藻酸钠包埋1.0×109/ml的绿色木霉的孢子溶液,用8号针头滴入3%CaCl2溶液中固化5h,缓冲液冲洗抽滤后。取10g固定化菌放入50ml/250mlpH为4.5的产酶发酵培养基的三角瓶中,在31℃、180r/min的条件下培养96h可达到最佳固定化效果,菌体经固定化后其耐高温性和耐热稳定性得到较大提高;重复使用六次后产酶率保持在80%左右。

The immobilization of Trichoderma viride in sodium alginate, we optimized the best immobilized conditions as follows： The concentration of inoculation spore suspension in 3.0%, alginate sodium was 1.0 × 10^9 spores/rnl. It was poured into 3.0% of CaCl2 solution. After solidification in the solution for 5 hours, pH 4.5 and span 96 hours for shaking flask culture at 31 ℃, 180 r/min.