摘要
用碱液从白木通(Akebiatrifoliate)茎中提取所得的粗多糖ATB经DEAECelulose及SephadexG200柱层析后得到一多糖纯品ATBB2,其分子量为23×105。糖组分分析ATBB2中各糖残基的摩尔比为Rha∶Ara∶Xyl∶Gal∶Glc∶GalA=122∶100∶110∶085∶024∶082。经甲基化,高碘酸氧化,Smith降解,部分酸水解,1H和13CNMR谱的分析揭示ATBB2是一个结构复杂的杂多糖。
Crude polysaccharide ATB was extracted from the stems of Akebia trifoliate with 5% NaOH. It was then subjected to DEAE Cellulose column chromatography and purified by Sephadex G 200 chromatography. Finally, a pure polysaccharide ATBB 2 was obtained. The molecular weight of ATBB 2 was estimated to be 2 3×10 5. By total acid hydrolysis, the analytical results indicated that ATBB 2 is composed of Rha, Ara, Xyl, Gal, Glc and GalA in a molar ratio of 1 22∶1 00∶1 10∶0 85∶0 24∶0 82. Methylation analysis, periodate oxidation analysis, Smith degradation, partial acid hydrolysis, 1H and 13 CNMR showed that ATBB 2 is a highly branched molecule without a main chain.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第6期438-441,共4页
Acta Pharmaceutica Sinica