RP-HPLC测定当药中环烯醚萜和三萜类成分含量

Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC

目的：建立应用RP-HPLC测定当药及不同部位中獐牙菜苦苷、当药苷、龙胆苦苷和齐墩果酸的含量测定方法。方法：采用ZORBAX SB-C18（4．6mm×250mm，5μm）色谱柱，流动相分别为乙腈-水（10：90）和甲醇-水（86：14），流速1．0mL·min^-1，检测波长分别为獐牙菜苦苷238nm、当药苷246nm、龙胆苦苷274nm和齐墩果酸207nm，柱温25℃。结果：獐牙菜苦苷、当药苷、龙胆苦苷和齐墩果酸分别在0．0342～0．4275，0．0011～0．0140，0．0007～0．0088，0．0011～0．0088mg·mL^-1线性关系良好，萨分别为0．9992，0．9998，0．9999，0．9996。结论：本法方便、准确，可用于当药药材的质量控制。

Objective： To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis. Method： A Zorbax SB-C18（4.6 mm ×250 mm,5 μm） column was used with acetonitrile-water（ 10： 90） and methnol-water（86： 14）at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin,sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL · min^-1 and the column temperature was 25℃. Result： All of the compounds were based - isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0. 344 4（r^2 =0.999 2）, 0.001 1-0.014 0（r^2 =0.999 8）, 0.001 1-0.013 4（r^2 =0.999 9）and 0.001 1-0.008 8 mg · mL^- l （ r^2 = 0.999 6）, respectively. Conclusion： The method is simple and accurate, which can be used for quality control of S. pseudochinesis.