摘要
目的:建立应用RP-HPLC测定当药及不同部位中獐牙菜苦苷、当药苷、龙胆苦苷和齐墩果酸的含量测定方法。方法:采用ZORBAX SB-C18(4.6mm×250mm,5μm)色谱柱,流动相分别为乙腈-水(10:90)和甲醇-水(86:14),流速1.0mL·min^-1,检测波长分别为獐牙菜苦苷238nm、当药苷246nm、龙胆苦苷274nm和齐墩果酸207nm,柱温25℃。结果:獐牙菜苦苷、当药苷、龙胆苦苷和齐墩果酸分别在0.0342~0.4275,0.0011~0.0140,0.0007~0.0088,0.0011~0.0088mg·mL^-1线性关系良好,萨分别为0.9992,0.9998,0.9999,0.9996。结论:本法方便、准确,可用于当药药材的质量控制。
Objective: To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis. Method: A Zorbax SB-C18(4.6 mm ×250 mm,5 μm) column was used with acetonitrile-water( 10: 90) and methnol-water(86: 14)at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin,sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL · min^-1 and the column temperature was 25℃. Result: All of the compounds were based - isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0. 344 4(r^2 =0.999 2), 0.001 1-0.014 0(r^2 =0.999 8), 0.001 1-0.013 4(r^2 =0.999 9)and 0.001 1-0.008 8 mg · mL^- l ( r^2 = 0.999 6), respectively. Conclusion: The method is simple and accurate, which can be used for quality control of S. pseudochinesis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第23期2494-2496,共3页
China Journal of Chinese Materia Medica