不同中药复方有效部位组合对脑缺血大鼠血浆中葛根素代谢变化的研究

Study on effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats

目的：建立HPLC测定血浆中的葛根素的方法，观测养阴通脑颗粒中主要有效部位的不同组合对脑缺血大鼠血浆中葛根素代谢变化的影响。方法：以乙腈提取处理血浆，以对羟基苯甲酸为内标，流动相甲醇-0．5％醋酸水（28：72），ODSC18色谱柱（4．6mm×150mm，5μm）为固定相，以紫外检测器在248nm测定血浆中葛根素的吸收。结果：葛根素在0．025—3．2μg·mL^-1线性关系良好，RSD〈10％，平均回收率为97．6％；有效部位配伍不同，血浆中葛根素的代谢变化不同。结论：此法灵敏、可靠，可较好地用于口服中药复方养阴通脑颗粒主要有效部位组合后血浆中葛根素的测定，可为有效成分的体内分析提供方法；不同配伍可影响成分的体内变化。

Objective： To build an experimental method to determine the content of puerarin in plasma, observe the effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats suffering cerebral ischemia attack after being orally administered the main active parts of Yangyin Tongnao granules. Method： Absorption of puerarin in plasma was extracted by acetonitrile. Using hydroxybenzoate as interior standard, methanol-0. 5% acetic acid water solution as mobile phase, ODS Cls chromatography column （4.6 mm× 150 mm, 5 μm） as stationary phase, puerarin was detected at 248 nm. Resuit： There is a good linear relationship for puerarin in the range 0. 025-3.2 μg· mL^-1 , RSD 〈 10%, and the average recovery is 97.6%. Conclusion： The method is sensitive, reliable, and can be well applied in this experiment. It can provide a method for determination of active components in TCM preparation in vitro. Different compatibility influences metabolism of components.