黄芪总苷及黄芪甲苷对糖皮质激素诱导衰老大鼠氧自由基代谢的影响

Effects of AST and ASI on metabolism of free radical in senescent rats treated by HC

目的:探讨黄芪总苷(astragaloside,AST)及黄芪甲苷(astragalus saponin I,ASI)对糖皮质激素诱导老前期大鼠衰老模型的延缓衰老作用及自由基代谢和免疫调节的影响。方法:用氢化可的松((hydrocorisone,HC,10mg.kg-1,sc,21 d)建立老前期大鼠的衰老模型,用Y迷宫检测大鼠的学习记忆功能;采用试剂盒检测衰老大鼠的肝和脑中MDA含量、SOD和CAT的活性以及GSH和GSSG的含量;采用Con A诱导测定大鼠脾淋巴细胞增殖反应,采用大鼠脾细胞增殖法测定IL-2活性。结果:与模型组比较,AST和ASI能改善HC诱导老前期大鼠衰老模型组大鼠的记忆功能,能降低肝和脑中MDA和GSSG含量,升高GSH含量,升高SOD和CAT活性,促进脾淋巴细胞增殖和IL-2活性。结论:HC可以促进老前期大鼠衰老,AST和ASI对HC诱导老前期大鼠衰老具有延缓作用,其机制可能与其抗氧化作用和调节免疫有关。

Objective： To study the effects of Astragaloside （AST） and Astragalus Saponin I （ASI） on metabolism of free radical and immune function in senescent rats treated by HC. Method： Hydrocorisone （ HC ） was used to estabilsh the aging model in rats. The content of molondialdehyde （MDA）, glutathoine （GSH） and oxidized glutathoine （GSSG） in liver and brain was detected according to kit. The activity of Mn superoride dismulase （Mn-SOD） and catalase （CAT） was also surveyed by kit. Concanavalin （ConA） was used to detect the proliferation and interleukin-2 （IL-2） production of splenocytes. Result： Compaired with HC control, AST and ASI could decrease the content of MDA, GSH and GSSG in liver and brain, increase the activity of Mn-SOD and CAT, and promote the proliferation and interleukin-2 （IL-2） activity of splenocytes. Conclusion： AST and ASI could delay the aging effect in rats treated by HC, and its mechanism maybe the antioxidant and regulating immunity.