踝三针对腰椎间盘突出根性痛大鼠中枢镇痛递质的影响

Effect of Huaisanzhen on central analgesic transmitters in the rat of the nerve root pain caused by protrusion of lumbar intervertebra disc

目的:探讨踝三针对腰椎间盘突出根性痛镇痛作用的机制。方法:雄性SD大鼠60只随机分为正常对照组、假手术对照组、模型对照组、来比林对照组、常规体针组、踝三针治疗组,分别采用肌肉注射药物来比林、常规穴针刺、踝三针穴位针刺治疗。测各组机械痛阈值和5-羟色胺(5-HT)含量。结果:大鼠痛阈值踝三针治疗组与模型对照组同时点比较显著增高(P<0.05);30 min时点痛阈值来比林对照组、常规体针组显著高于模型对照组,踝三针治疗组与来比林对照组、常规体针组比显著增加(均P<0.05)。5-HT测量值踝三针治疗组与模型对照组、来比林对照组在相同时点比较差异具有非常显著性意义(P<0.01);30 min时点5-HT测量值踝三针治疗组显著高于常规体针组(P<0.05)。结论:踝三针镇痛作用与脑内5-HT递质含量增加密切相关。

Objective To search the mechanism of Huaisanzhen in treating the nerve root pain caused by protrusion of lumbar intervertebra disc. Methods Sixty male SD rats were randomly divided into a normal control group, a sham operation control group, a model control group, an aspirin-DL-lysine control group, a routine body acupuncture group, and a Huaisanzhen treatment group. The aspirin-DL-lysine control group, the routine body acupuncture group and the Huaisanzhen treatment group were treated by aspirin-DL-lysine through intramuscular injection, routine acupuncture and Huaisanzhen therapy, respectively. The pain threshold and the content of brain 5-HT in each group were determined. Results The pain threshold significantly increased in the Huaisanzhen treatment group as compared with that in the model control group at the same hour points （P〈0. 05）. And at the 30 min, the pain threshold in the aspirin-DL-lysine control group and the routine body acupuncture group was significantly higher than that in the model control group, and the Huaisanzhen treatment group was significantly higher than that in the aspirin-DL-lysine control group and the routine body acupuncture group （all P〈0. 05）. The 5-HT contents at the same time in the Huaisanzhen treatment group were higher than those of the model control group and the aspirinDL-lysine control group （P〈0. 01）. The 5-HT content at 30 min in the Huaisanzhen treatment groups was significantly higher than that of the routine body acupuncture group （P〈0. 05）. Conclusion The analgesic effect of Huaisanzhen treatment is closely related to increase of 5-HT content in the brain.