Survivin基因与NET-1蛋白在膀胱癌中的表达及相互关系

Relationship between expression of survivin gene and NET-1 protein in bladder carcinoma

目的:探讨survivin基因和NET-1蛋白在膀胱癌中的表达及其与临床病理特征的关系。方法:采用逆转录聚合酶链反应和免疫组化方法分别检测27例膀胱癌组织和10例正常膀胱组织survivin基因和NET-1蛋白的表达,并将结果进行相关分析。结果:27例膀胱癌组织中survivin基因阳性17例(62.96%),NET-1蛋白阳性21例(77.78%);10例正常膀胱组织未见survivin基因表达,NET-1蛋白表达10.00%(1/10),二者差异有统计学意义(P<0.01);survivin基因与NET-1蛋白表达有相关性(χ2=7.09,P<0.01),survivin基因和NET-1蛋白表达与膀胱癌WHO分级和TNM分期有关,与患者年龄和性别无关。结论:survivin基因和NET-1蛋白在膀胱癌中过度表达表明膀胱癌发生、发展与细胞凋亡和细胞增殖有关,survivin基因对膀胱癌早期诊断和靶向治疗有重要意义。

Objective： To investigate the relationship between the expression of survivin gene and NET-1 protein in bladder Carcinoma. Methods： By reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemical assay （SP）, the expression of survivin gene and NET-1 protein were detected in 10 eases of normal tissues and 27 cases of bladder carcinoma tissues. The results Were analyzed by corresponding statistica methods. Results： Survivin gene and NET-1 protein showed higher expression in bladder carcinoma. The rate of positive expressions were 62.96% and 77.78%, while the positive rates were 0% and 10% in nomal tissues. The difference was of significance between the two tissues （P〈0.01）. The expression of survivin gene correlated with NET-1 protein expression（X2=7.09, P〈0.01 ）. The expressions of survivin gene and NET-1 protein were correlated with WHO pathology classification,TNM stage and prognosis（P〈0.01）, and were ineorrelated with age and sex. Conclusions： Both survivin gene and NET-1 protein were highly expressed in bladder carcinoma, which suggests that the ocurrence of bladder carcinoma occur was correlated with cellular apoptosis and proliferation. Survivin gene might play roles for clinical early diagnosis and target treatment of bladder carcinoma.