中国北方汉族CD14启动子C(-260)T基因点突变及血浆IL-6含量与冠心病的相关性

Study of the relationship between CD14 gene promoter C(-260) T point mutation and plasma IL-6 and coronary artery disease in northern Chinese Han population

目的探讨冠心病(CHD)患者CD14启动子C(-260)T基因点突变情况及血浆IL-6含量变化与CHD的相关性。方法采集115例CHD病人和60例健康人抗凝血,用PCR-RFLP法测定CD14启动子C(-260)T基因点突变情况,ELISA法检测血浆IL-6含量。结果CHD组CD14启动子C(-260)T基因点突变频率与对照组无统计学差异(χ2=2.644,P=0.267);CHD组血浆IL-6水平高于健康对照组(t=3.553,P<0.01);CHD病人不同基因型组血浆IL-6含量无统计学差异(F=0.294,P=0.749)。结论CD14启动子C(-260)T基因多态性可能不是CHD的基因决定性危险因子,但作为炎性因子的IL-6在CHD的发生、发展中起着重要的作用,

Objective To detect the point mutation of CD14 gene promoter C （-260） T and plasma level of IL-6 to explore the possible pathogenic mechanism of coronary heart disease （CHD）, and to analyze the association between plasma IL-6 levels and genotypes of CD14 and CHD. Methods The point mutation of CD14 gene promoter C （-260） T were analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） methods in 115 CHD patients and 60 healthy controls. IL-6 content was assayed by ELISA. Results There was no significant difference in CD14 gene promoter C （-260） T polymorphism between CHDs and controls （X^2 = 2. 644, P = 0. 267）. Plasma level of IL-6 were high in CHDs than that in controls （ t = 3. 553, P 〈 0. 01 ）. There were no significant differences in plasma level of IL-6 among the genotypes of CDI4 gene promoter C （ - 260） T in CHDs （ F = 0. 294 ,P = 0. 749）. Conclusions CD14 gene promoter C （-260） T may not be the main risk factor of CHD, while IL-6, as an inflammatory factor, plays an important role in the trigger and develonment of CHD.