摘要
从驯鹿皱胃组织中提取总RNA,根据已发表的驯鹿生长素Ghrelin基因序列设计并合成引物,通过反转录-聚合酶链式反应(RT-PCR)进行cDNA扩增,获得了300 bp的片段,重组到pBlueselect T载体,经限制性内切酶谱分析和DNA序列测定分析,确认PCR产物为Ghrelin cDNA,为进一步研究Chrelin在驯鹿体内的分布及营养因素等对其基因表达的影响奠定基础。
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor （GHS-R）, is a kind of muhi-peptide of 28 amino acid residues and can facilitate to release of GH. Total RNA was extracted from the abomasus tissue of reindeer and 300 bp gene fragment was obtained by RToPCR with the primers which had been designed according to the published reindeer ghrelin gene sequence and synthe- sized. After being cloned into pBlueselect T vector, the PCR segment was determined as ghrelin cDNA by restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing.
出处
《经济动物学报》
CAS
2007年第4期196-198,210,共4页
Journal of Economic Animal
基金
内蒙古农业大学博士启动基金资助项目(BJ04-2)
