血管性抑郁大鼠模型的建立

Establishment of Rat Model of Vascular Depression

目的探讨建立动脉硬化叠加抑郁动物模型,为血管性抑郁症的深入研究创造条件。方法选择SD大鼠,采用维生素D3(VD3)肌肉注射(30万U/kg,连续3 d)加高脂饲料饲养9周,在此基础上给予慢性不可预见性温和应激刺激(CUMS)3周,进行动脉硬化叠加CUMS抑郁造模。检查体质量和血脂变化,用敞箱试验评价大鼠活动度和好奇心,用糖水、纯水摄入量评价大鼠快感缺乏与否。结果与对照组相比,模型组大鼠体质量下降;血脂检查中,胆固醇(CHO)、低密度脂蛋白(LDL)升高,高密度脂蛋白(HDL)降低,差异均有统计学意义(P<0.05);敞箱实验中,垂直得分、水平得分、总分均显著降低,差异有统计学意义(P<0.01);糖水、纯水摄入量测定实验中,糖水百分比降低,纯水摄入量显著升高,差异有统计学意义(P<0.05)。结论VD3肌肉注射加高脂饲料饲养可复制动脉硬化大鼠模型,叠加CUMS,可兼具高脂血症和抑郁两方面的症状,与人血管性抑郁症的临床表现有较好的相似性,是较理想的血管性抑郁症动物模型。

Objective To explore the establishment of animal model of arteriosclerosis combined with depression.Methods Arteriosclerosis combined with depression model was established by injecting intramuscularly with vitamin D3 300 000U/kg for 3 days,feeding with high lipid feed for 9 weeks and followed by chronic unpredictable mild stress（CUMS） for 3 weeks.The model was evaluated with weight,blood fat,activity and interest（Open-field test） and hedonia（test of 1% sucrose solution and pure water intake）.Results Comparing with the control group,the model group has four results.1.the weight decreased markedly（t=-5.591,P〈0.001）.2.CHO and LDL increased markedly（t=5.31,P〈0.001,t=5.943,P〈0.001）,the HDL decreased markedly（t=-2.118,P〈0.05）.3.the vertical scores,horizontal scores and total scores in Open-field test decreased markedly（t=-3.989,P〈0.01,t=-6.368,P〈0.001,t=-6.254,P〈0.001）,4.Intake of 1% sucrose solution percentage decreased markedly（t=-2.296,P〈0.05） and the pure water intake increased markedly（t=2.397,P〈0.05）.Conclusion This model was characterized with weight,HDL of blood,activity,interest and hedonia decreased obviously and CHO,LDL of blood and pure water intake increased markedly,which has the symptoms of both hyperlipidemia and depression.So,the model,which established by injecting intramuscularly with vitamin,feeding with high lipid feed and followed by chronic unpredictable mild stress（CUMS）,is similar to clinical manifestation of vascular depression in human.It is an appropriate model of vascular depression.