摘要
目的研究从大鼠附睾处脂肪垫组织中分离到的间充质干细胞在体外诱导为软骨细胞的生物学特性。方法取SD大鼠附睾处脂肪垫组织,酶消化分离获间充质干细胞,经诱导液在体外诱导6周,观察、检测软骨细胞的生物学特性。结果大鼠脂肪组织来源的间充质干细胞,诱导5d后倒置显微镜下见形态发生变化;检测到蛋白聚糖,随诱导时间延长含量呈增加趋势;Ⅱ型胶原单克隆抗体免疫荧光染色阳性,逆转录聚合酶链反应从RNA水平验证了Ⅱ型胶原的表达。结论大鼠脂肪组织来源的间充质干细胞可在体外定向诱导表达为软骨细胞。
Objective To study the biological characteristics of the chondrocytes differentiated in vitro from mesenchymal stem cells derived from Sprague-Dawley rat epididymal fat pads. Methods The adipose tissues-derived mesenchymal stem cells were separated from the epididymal fat pads of the rats, and then the biologic characters of the stem cells were observed and tested after in vitro induction by culturing with a condictional medium for six weeks. Results Morphological changes of the adipose mesenchymal stem cells were observed after in vitro induction in condictional medium for 5 days. The chondrocytes induced from the adipose tissue mesenchymal stem cells secreted aggrecan in an increased amount with time,and the secretion of collagen type Ⅱ antigen was detected by indirect immunoflurescence methods. The expression of collagen type Ⅱ was also confirmed by reverse transcription polymerase chain reaction analysis. Conclusion Adipose tissue-derived mesenchymal stem cells may be induced to express chondrogenic markers under in vitro culture with a conditional medium.
出处
《江苏医药》
CAS
CSCD
北大核心
2007年第12期1239-1241,F0002,共4页
Jiangsu Medical Journal
基金
江苏省卫生厅135工程资助(38RC2002038)
苏州市科技发展规划课题(SSY0630)
