血管内皮细胞生长因子对骨缺损修复的影响

Effects of vascular endothelial growth factor for repairing bone defect

[目的]探讨血管内皮细胞生长因子（VEGF）对肌肉骨膜瓣、松质骨颗粒和骨髓联合移植修复骨缺损中的影响．[方法]制作家兔桡骨骨缺损模型，在缺损区用肌肉骨膜瓣、松质骨颗粒及骨髓进行联合移植，于术后第1、2、4、6、8周时利用光学显微镜和免疫组织化学等方法检测VEGF在骨缺损修复不同阶段中对各种细胞内的表达情况．[结果]VEGF在骨缺损修复过程中的不同阶段的表达程度不同，在术后1～6周时（即软骨内化骨阶段），新生的骨细胞、成骨细胞及软骨细胞内均可见大量的VEGF，其高峰出现在第4周[结论]VEGF是骨缺损修复过程中重要的生长因子，联合移植有利于骨缺损的快速修复．

OBJECTIVE To study the expression and effects of vascular endothelial growth factor （VEGF） for combined transplanting the musculoperiosteal flap, bone marrow and spongy bone granules to repairing bone defect. METHODS Segmental bone defect was created in the mid upper of radial shaft of adult rabbits, and combination of musculoperiosteal flap, bone marrow and spongy bone granules was transplanted to the bone defect. VEGF expression in the different phase of repairing bone defect was defected by immunohistochemistry at postoperative of l, 2, 4, 6, 8 weeks. RESULTS Expression of VEGF was different in the different phase of repairing bone defect. VEGF was expressed in neo-osteocytes, osteoblasts and chondrocytes in the bone defects sites from one to six weeks （phase of os endochondrale） after operation, and the high expression of VEGF was detected at 4 weeks. CONCLUSION VEGF is key factor in healing bone defects, and combined transplantation makes for repairing the bone defect.