内皮脂酶对人脐静脉内皮细胞黏附分子表达的影响

Effects of endothelial lipase on mRNA expression of adhesion molecule of human umbilical vascular endothelial cells

目的研究人脐静脉内皮细胞（HUVECs）表达黏附分子与内皮脂酶（EL）的关系，以及EL对内皮细胞黏附分子表达的影响。方法以肿瘤坏死因子-α（TNF-α）10μg／L刺激HUVECs，采用逆转录-聚合酶链反应（RT—PCR）检测细胞间黏附分子-1（ICAM-1）、血管内皮黏附分子-1（VCAM-1）和E-选择素的mRNA表达变化，以50μg／L的抗EL抗体进行预处理，再检测黏附分子mRNA表达的变化。利用凝胶成像系统拍照、分析、计算黏附分子产物相对含量。结果TNF-α作用于HUVECs后，黏附分子ICAM-1、VCAM-1和E-选择素的mRNA表达均增高，与正常对照组比较差异有统计学意义（P均〈0．01）；该作用可被50μg／L的抗EL抗体所拮抗（P〈0．05或P〈0．01）。结论EL参与了内皮细胞黏附分子表达的调控，推测它可能通过影响内皮细胞黏附分子表达而参与动脉粥样硬化的病理生理过程。

Objective To explore the relationship between human umbilical vascular endothelial cells （HUVECs） and endothelial lipase （EL）, and the effect of EL on expression of endothelial cell adhesion molecule （ICAM）. Methods HUVECs was treated with tumor necrosis factor-α （TNF-α） 10 μg/L and the mRNA of adhesion molecules [intercellular adhesion molecule - 1 （ICAM - 1）, vascular cellular adhesion molecule - 1 （VCAM - 1） and E -selectin] were detected by reverse transcription -polymerase chain reaction （RT-PCR）. Then the effect of 50 μg/L anti -endothelial lipase （anti- EL） antibody on the influence of TNF - α on these adhesion molecules was observed. Results After being treated with TNF - α, the mRNA of adhesion molecules expressed by HUVECs were significant up -regulated, there was significant difference compared with control group （all P〈 0.01）. These effects of TNF- α were significantly abolished by 50 μg/L anti -EL antibody （P〈0.05 or P〈0.01）. Conclusion EL can affect the expression of adhesion molecules on endothelial cell adhesion molecule. This effect of EL may play a role in the pathphysiologic process in the pathogenesis progress of atherosclerosis.