重症急性胰腺炎大鼠早期肺组织损伤的实验研究

Experimental study on early pulmonary injury induced by severe acute pancreatitis in rats

目的观察重症急性胰腺炎（SAP）早期对肺组织损伤的影响。方法将健康成年雄性SD大鼠40只随机分成假手术组及SAP后30、60、120和360min组，每组8只大鼠。采用经胰胆管逆行注入质量分数为5％的牛磺胆酸钠溶液的方法来制备SAP模型。于各时间点活杀大鼠，分别观察大鼠动脉血气分析、肺组织病理改变及检测肺组织湿／干重（W／D）比值。结果SAP大鼠360min时肺组织内即有大量中性粒细胞浸润和显著的病理学改变，且肺组织含水量明显增多、剩余碱（BE）值显著下降（P〈0．05或P〈0．01），但对大鼠动脉血氧分压（PaO2）和动脉血二氧化碳分压（PaCO2）的变化影响不明显（P均〉0．05）。结论SAP早期即可造成大鼠肺组织显著的炎症反应，且肺组织存在一定程度的损伤。

Objective To investigate the injury to pulmonary tissue induced by severe acute pancreatitis （SAP） during its early period. Methods Forty adult male Sprague -Dawley （SD） rats weighing 300 - 400 g were randomly divided into 4 groups with 8 animals in each group： sham operation group and 30, 60, 120 and 360 minutes after SAP groups. The model of SAP was reproduced by retrograde injection of 5% sodium taurocholate into the pancreatic duct of rats. Rats were sacrificed at different time points, and blood gas analysis, pulmonary pathological changes and wet dry weight ratio （W/D） of the lung were conducted. Results A large amount of polymorphonuclear neutrophils were observed in pulmonary tissue within 360 minutes after reproduction of SAP with obvious changes, and water content of pulmonary tissue was increased significantly, while base excess （BE） decreased significantly in rats with SAP （P 〈 0. 05 or P〈0.01）. But no obvious change was observed in arterial partial pressure of oxygen （PaO2） and partial pressure of carbon dioxide （PaCO2, both P〉0.05）. Conclusion SAP causes significant inflammation in rat lung with injury to pulmonary tissue in its early period .