吉林地区伯氏疏螺旋体OSPA的克隆及免疫性的初步研究

Molecular cloning of Borrelia burgdorferi's OSPA in Jilin and preliminary research on the protectivity of OSPA

目的克隆吉林地区莱姆病螺旋体分离株外膜蛋白A(OSPA),并对其免疫性进行初步研究,为进一步研制莱姆病疫苗提供基础。方法用聚合酶链反应(PCR)从吉林地区莱姆病螺旋体分离株的全基因组DNA中将OSPA基因调出,插入载体pCDNA3.1(+)中,构建重组质粒pCDNA3.1(+)-OSPA,用重组pCDNA3.1(+)-OSPA质粒免疫C57小鼠,用ELISA法检测其血清特异性抗体,从而对其免疫性有初步的了解。结果pCDNA3.1(+)-OSPA重组质粒构建成功,用pCDNA3.1(+)-OSPA重组质粒免疫C57小鼠,在其血清中可检测到特异性抗体。结论成功地对吉林地区莱姆病螺旋体分离株的OSPA基因进行了克隆,OSPA有较好的免疫原性,可作为莱姆病疫苗的一种有效成分。

Objective Cloning OSPA gene from a Jilin province＇s Borrelia burgdofferi strain, and researetfing on the proteefivity of OSPA for vaccine development in China. Methods PCR and gene recombination technique were used to done the OSPA gene from a Borrelia burgdofferi strain. Then the OSPA gene was inserted into vector pCDNA3.1 （ ＋ ）. The recombinant ptasmid pCDNA3.1 （ ＋ ）-OSPA was used to immunized C57 mice. After mice were immunized by recombinant plasmid pCDNA3.1 （ ＋ ）-OSPA, the antibody in serum of the mice was tested by ELISA. Results The recombinant plasmid pCDNA3.1 （ ＋ ）-OSPA was founded successfully.The ELISA＇ s results of the mice inununized with pCDNA3.1 （ ＋ ）-OSPA was positive. Conclusion OSPA gene from a Jilin province＇s Borrefia burgdoKeri strain was successfully cloned. The OSPA has strong immunity, so it may be used as the part of vaccine for Lyme disease.