单纯疱疹病毒Ⅰ型糖蛋白B核酸疫苗对小鼠血液学指标的影响

The influence of herpes simplex virusⅠ-glycoprotein B DNA vaccine to the index of mice hematology

目的检测单纯疱疹病毒Ⅰ型糖蛋白B核酸疫苗(pcDNA3-gB)对小鼠血液生理学和血液生化学指标的影响,探索其安全性及实用性。方法将不同浓度pcDNA3-gB(1 mg/ml3、mg/ml5、mg/ml)通过肌肉注射免疫健康小鼠,对照组使用等量生理盐水,每10天免疫一次,共3次。末次免疫后24 h,检测各组小鼠9项血常规指标和9项血液生化学指标。结果各组小鼠的全部血液生理、生化指标均较稳定,各试验组与对照组之间均无显著差异(P>0.05)。结论不同剂量pcDNA3-gB免疫小鼠后,对小鼠的血液生理生化指标均无明显影响,初步证实了其安全性。

Objective To detect the influence of herpes simplex virus 1 -glycoprotein B DNA vaccine to the index of mice hematology.Methods Twenty-four mice were divided into four groups randomly. The four groups were control group, 1 mg/ml,3 mg/ml、 5 mg/ml DNA vaccine group. There were six mice in each group and a half of them were male,and others were female. To inject mice different dose of DNA vaccine every ten days, and give normal saline to control group. Totally injections were three times. After the last injection, we killed mice and collected blood to examine the index of their hematology. Results Compare to the control group, index of other groups were not found differences obviously in statistics（ P 〉 0.05）. Conclusion Because there were no obvious differences between each two groups, we consider the herpes simplex virus Ⅰ -glycoproteinB DNA vaccine Ires no influence to the index of mice hematology.