肝硬化患者红细胞免疫功能与内毒素、TNF-α、NO水平的相关研究

Study on the Relationship Between Red Blood Cell Immunofunction and Serum Endotoxin (LPS),TNF-α,NO Levels in Patients with Liver Cirrhosis

目的:探讨了肝硬化患者红细胞免疫功能与内毒素(LPS)、肿瘤坏死因子α(TNF-α)和一氧化氮(NO)水平的关系。方法:应用红细胞酵母花环法测定红细胞免疫功能,放免法测定TNF-α,生化法测定LPS和NO。对71例肝硬化患者进行了红细胞免疫功能和LPS、TNF-α、NO水平测定,并与35名正常健康人作比较。结果:肝硬化患者RBC-C3b水平明显低于正常人组(P<0.01),而RBC-ICR、TNF-α、LPS、NO水平明显高于正常人组(P<0.01),相关分析显示,RBC-C3b水平与LPS、TNF-α、NO水平呈明显负相关(r=-0.4129、-0.3928、-0.4012,P<0.01)。结论:肝硬化患者红细胞免疫黏附功能的降低与LPS、TNF-α和NO水平密切相关。

Objective To study the relationship between red blood cell Immunofunction and serum endotoxin （ LPS）, tumor necrods factor - α（TNF - α）, nitric oxide （NO） levels in patients with liver cirrhosis. Methods The red blood cell C3b receptor （ RBC -C3b） and RBC immunocomplex receptor （RBC -ICR） percentages （with yeast rosettes）, serum TNF - α （with RIA）. LPS and NO （with biochemistry） levels were measured in 71 patients with liver cirrhosis and 35 controls. Results RBC - C3b rosette percentages in the patients were significantly lower than those in controls （ P 〈 0.01 ）. While the RBC - ICR percentages, LPS, TNF - α and NO leveh were significantly higher （P 〈 0.01 ）. RBC - C3b percentages were negatively correlated with serum LPS, TNF - α and NO leveh （r= -0.4129, -0. 3928, -0.4012,P〈0. 01）. Conclusion The lowered red cell immuno- function in patients with liver cirrhosis was closely associated with increase of serum LPS, TNF - α, NO levels.