血浆明胶酶检测在慢性肾脏疾病中的临床意义

The Clinical Implications of Changes of Circulating Matrix Metalloproteinase-2 and -9 Levels in Patients with Chronic Kidney Disease

目的:通过观察慢性肾脏病(CKD)患者血清MMP2,MMP9的变化与血肌酐的关系,探讨对CKD患者行MMPs检测的临床意义。方法:用ELISA法对60例CKD患者和40例健康对照组的血清MMP2、MMP9进行检测,分析CKD患者血清MMP2、MMP9与血肌酐(Scr)的相关性。结果:CKD患者血清MMP2、Scr显著高于对照组(P<0.001),而血清MMP9、eGFR显著低于对照组(P<0.05)。CKD患者Scr与血清MMP2正相关(r=0.276,P<0.05),与血清MMP9负相关(r=-0.389,P<0.01)。结论:CKD患者血清MMP2升高,MMP9降低,可能促进CKD的进展。

Objective To investigate the clinical implications of changes of changes of circulating MMP - 2 and MMP - 9 levels in patients with CKD. Mothods Plasma MMP - 2 and MMP - 9 activities were measured with ELISA in 60 CKD patients and 40 controls. The blood ereatinine levels, urinary albumin contents were also determined and eGFR were calculated in these patients. Results Serum ereatinine concentrations and MMP - 2 activities were significantly higher （P 〈 0.001 ） while MMP - 9 activity and evaluated glomerular filtration rate（eGFR） were significantly lower （P 〈0.001） in CKD patients than the respective values of controls. In addition, serum creatinine concentrations were positively correlated with MMP - 2 activity （ r = 0. 276, P 〈 0.05 ） and negatively correlated with MMP - 9 activity （ r = 0. 389, P 〈 0.01）. Conclusion This study demomtrated a correlation between MMP - 2, MMP - 9 and serum creatinine in CKD patients, suggesting that MMP -2 and MMP -9 might play some role in the pathogenesis of CKD.