血清TIMP-1测定在肝炎、肝硬化中的诊断价值

Diagnostic Value of Determination of Serum Tissue Inhibitor of Matrix Metallo-Proteinase-1 (TIMP-1) Levels in Patients with Hepatitis and Cirrhosis

目的:了解肝炎、肝硬化患者基质金属蛋白酶抑制因子(TIMP-1)在血清中的表达以及肝纤维化的情况,探讨肝炎致肝硬化的机理。方法:用ELISA检测TIMP-1在肝炎、肝硬化患者血清中的含量,同时检测HA、PCⅢ、CⅣ、LN。结果:肝硬化患者血清中TIMP-1的含量与肝炎组相比显著增加,TIMP-1与HA、PCⅢ、CⅣ、LN呈明显正相关。结论:TIMP-1在血清中的含量可判断肝脏纤维化的程度,为肝硬化的诊断提供实验依据。

Objective To investigate thd relationship between the degree of hepatic fibrosis and serum expression of tissue inhibi- tor of matrix metalloproteinase - 1 （ TIMP - 1 ） in Patients with hepatitis and cirrhosis. Methods Serum TIMP - 1 （ with ELISA） and hepatic fibrosis markers HA, PC Ⅲ, C Ⅳ, LN （ with KIA） contents were measured in 33 patients with hepatic cirrhosis and 30 patients with viral hepatitis. Results The serum levels of TIMP - 1 in patients with hepatic cirrhosis were significantly higher than those in patients with hepatitis. Levels of TIMP - 1 were significantly positively correlated with levels of the hepatic fibrosis markers HA, PC Ⅲ, CⅣ and LN. Conclusion TIMP - 1 could be used as a marker of hepatic fibrosis and could be helpful for diagnosis of cirrhosis.