乙型肝炎病毒基因分型荧光PCR检测及临床应用

Clinical Application of HBV Genotyping with Fluorescence PCR

目的:了解乙型肝炎病毒基因分型、分布及其与肝功能损害、病毒复制水平的关系。方法:采集69例乙肝患者血样,采用荧光聚合酶链反应(PCR)检测乙肝病毒基因分型,通过荧光探针识别基因型特异性序列,采集分析荧光信号确定病毒基因型,分别检测谷丙转氨酶(ALT)水平、乙肝病毒e抗原(HBeAg)和HBV DNA含量。结果:69例乙肝患者中B型26例,C型43例。B型和C型患者的ALT水平分别为(453.54±447.93)U/L和(330.23±306.90)U/L(P>0.05);HBeAg阳性数分别为11例和23例(P>0.05);HBV DNA含量分别为(38.74±36.49)×104拷贝/ml和(34.53±34.09)×104拷贝/ml(P>0.05);不同临床类型乙肝患者中两种基因型的分布没有显著差异(P>0.05)。结论:本地区乙肝患者病毒基因型主要为B型和C型;二种基因型ALT水平、HBeAg表达水平、病毒复制水平以及肝炎病情均无显著性差异。

Objective To study the possible relationship between HBV genotype and degree of impairment of liver function as well as levels of virus rephcation. Methods HBV genotyping with fluorescence PCR was performed in 69 patients with various types of HBV hepatitis. The serum ALT levels, HBeAg positivity and HBV - DNA contents were also determined. Results Among the 69 patients, 26 were of genotype B and 43 were of genotype C. The ALT levels （452.54±447.93 vs 330.23±306.90U/L respectively）, HBeAg positive numbers （ 11 vs 23 ） and HBV - DNA contents （38.74±36.49×10^4 copies/ml vs 34.53±34.09×10^4 copies/ml） in these 2 groups of patients were not significantly different （ all P 〉 0.05）. Again, neither genotype B nor genotype C was more prevalent in any type of HBV hepatitis patients. Conclusion Local HBV hepatitis patients were of either B or C genotype, with no significant difference clinically.