当归注射液对坐骨神经痛小鼠脊髓星形胶质细胞活性的影响

Effects of Radixange Sinensis on the Changes of Spinal Astrocytes Induced by Sciatic Neuralgia in Mice

目的:探讨当归注射液的镇痛机制。方法:24只昆明小鼠随机均分为假手术对照组、坐骨神经痛模型组(模型组)、坐骨神经痛模型+当归注射液6ml·kg-1组(给药组Ⅰ)和坐骨神经痛模型+当归注射液12ml·kg-1组(给药组Ⅱ)。各组在手术后第13天开始分别给予相同体积的0.9%氯化钠溶液、0.9%氯化钠溶液、当归注射液6ml·kg-1、当归注射液12ml·kg-1腹腔注射,每天8:00、17:00时给药2次,连续给药3d。在第6次给药后间隔lh取脊髓组织采用免疫组织化学方法测定脊髓星形胶质细胞胶质纤维酸性蛋白(GFAP)免疫反应阳性产物的变化,观察当归镇痛过程中是否对脊髓星形胶质细胞激活程度有影响。结果:(1)坐骨神经损伤后,脊髓受累节段星形胶质细胞发生显著改变,损伤15d后模型组可见活化的星形胶质细胞,且GFAP呈强阳性反应,可见慢性疼痛形成过程中脊髓GFAP免疫反应阳性产物数量增加;(2)模型组GFAP免疫反应阳性产物染色较对照组深,其阳性产物光密度值(0.167±0.023)与对照组光密度值(0.118±0.014)相比增加了91%(P<0.01);(3)给药组ⅡGFAP阳性产物光密度值比模型组降低22%,其差异有显著性(P<0.01)。结论:当归注射液镇痛机制可能涉及脊髓星形胶质细胞的变化,当归注射液可能通过抑制脊髓星形胶质细胞激活而具有部分镇痛作用。

Objective： To study the analgesia mechanism of radixange sinensis. Methods Twenty-four mice were divided randomly into the sham operated group ＋ physiological saline（the control group）, the chronic neuropathic pain group ＋ physiological saline（TS group）, the chronic neuropathic pain＋ radixange sinensis 6ml · kg^-1 group（TS＋ Ⅰ group）, the chro nic neuropathic pain＋radixange sinensis 12ml · kg^-1 group（TS＋Ⅱ group）. Chronic neuropathic pain was induced by tying off sciatic nerve of rats. Dmg or Vehicle 2ml was injected intraperioneal injection（ip. ）from the thirteenth day to the fifteenth day after surgery,twice a day（at 8：00 and 17：00, respectively）. The changes of the spinal astrocytes were observed by Immunohistochemistry ABC method in lh after the sixth intraperitoneal injection. We examined the effect of radixange sinensis,and determined whether radixange sinensis infected the degree of astrocyte activation or not. Result： The GFAP positive cells of TS groups showed stronger staining than control groups.