木蝴蝶药材HPLC指纹图谱研究

Studies on HPLC Fingerprint of Semen Oroxyli

目的：建立木蝴蝶药材的指纹图谱。方法：采用反相高效液相色谱法（RP-HPLC）,色谱条件为：Lichrospher C18（250mm×4.6 mm Media 10 nm,5μm）;流动相：A：1%醋酸乙睛,B：1%醋酸水溶液。洗脱方法：（0～30）min,乙腈%为10%～25%;（30～50）min,乙腈%为25%～55%,保持5 min;流速1.0 mL·min^-1,柱温：30℃。结果：测定了黄芩苷含量,构建了木蝴蝶药材的指纹图谱,对不同产地来源的木蝴蝶药材分类。结论：该法准确,重复性好,可作为木蝴蝶药材的质控方法。

Objective：To establish a HPLC fingerprint of Semen Oroxyli. Methods：The analysis was carried out on a Liehrospher C18 column（250 mm×4.6 mm, 5 μm） eluted with the mobile phase consisted of aeetonitfile（A） and water（B） all containing 1% acetic acid in gradient mode. The gradient program was as follows：0- 30 min, A changed from 10% - 25 % ; （30 - 50）min, A changed from 25 % to 55 % ; then the column keeped for 5 min. The flow rate was 1. 0 mL·min^-1. The wave length was 280 nm and temperature was 30 ℃. Result： The fingerpring of Semen Oroxyli was estabilished. Conclusion：The method is repeatable and can be used in quality control of Semen Oroxyli.