正交试验法优选玉屏风总苷的提取工艺

Study on the Extraction Process for Total Glucosides of Yupingfeng with Orthogonal Design

目的:优选玉屏风散中有效部位-玉屏风总苷(TGYPF)的提取工艺。方法:设计L9(34)正交试验,采用HPLC-ELSD法测定黄芪甲苷的含量作为质控指标,考察乙醇浓度(A)、乙醇用量(B)、提取时间(C)和提取次数(D)4个因素对玉屏风总苷(TGYPF)提取的影响,优化提取工艺。结果:最佳提取条件为:A2B3C3D2,即80%乙醇,8倍量,提取2次,每次3 h。结论:该提取工艺稳定性和重现性良好,简便,快速,可行。

Objective：To study the optimized extraction process of total glucosides of Yu Ping Feng（TGYPF）, one of the effective parts of Yupingfeng. Methods ： By use of orthogonal experimental design L9 （34 ） , four factors that may effect the extraction had been studied, including alcoholic concentration, alcoholic volume, extracting time and times. And the content of astragloside IV had been developed as its quality standard, screening out the best method of extraction conditions. Results：The best extracting technical conditions were as follows： 8 times amount of 80% EtOH, extractin： solvent to reflux for 3 hour and 2 times. Conclusion： The optimized process is simple, fast and feasible, with its stability and repeatability are good respectively.