柴荆注射液的解热作用机理研究——对下丘脑中精氨酸加压素及前列腺素E_2含量的影响

Studies on Antipyretic Mechanism of Chaijing Injection-interfere with Content of AVP and PGE_2 in Rat Hypothalamus

目的:研究柴荆注射液对酵母致热大鼠体温的影响及对下丘脑中精氨酸加压素(AVP)及前列腺素E2(PGE2)含量的影响,为其解热作用机理提供依据。方法:应用20%高活性酵母诱致大鼠发热,观察柴荆注射液的解热作用并用放射免疫检测法分析柴荆注射液对下丘脑AVP及PGE2含量的影响。结果:①皮下注射20%酵母可致大鼠发热,柴荆注射液具有明显的退热作用,退热效果高剂量组优于低剂量组;②能明显降低致热大鼠下丘脑中PGE2及AVP的含量,与对照组比较具有明显差异。结论:柴荆注射液能降低致热大鼠下丘脑中PGE2及AVP的含量,通过对下丘脑中分泌的这两个重要的内源性介质的抑制和释放利用,起到良好的退热作用。

Objective：To study the effect of Chaijing injection on in febrile rats induced by zymonsa and content of AVP as well as PGE2 in rat hypothalamus. Provide a scientific evidence for the antipyretic mechanism. Methods ： Rats febrific model were made by 20% high-activity zymonsa, the effective mechanism of Chaijing Injection was probed in antipyretic action on temperature and cut off rat hypothalamus, the radioimmunoassay （RIA） was established for the determination of AVP and PGF2 in hypothalamus. Results： （1）The rat was injected by way of subcutaneous with 20% zymonsa that could cause rat febrific model. The Chaijing Injection had a obviously antipyretic effect on febrile rats, the effect in 3 mL·kg^-1 group was remarkably better than that in 2 mL·kg^-1 group. （2）The content of AVP and PGE2 in hypothalamus of rats in treated group with Chaijing Injection had a significant lowering compared with the model control group. Conclusions： The content of AVP and PGF2 in hypothalamus of rats could be reduced by treated group with Chaijing Injection. Both AVP and PGE2 were important endogenic mediums that were secreted in hypothalamus, through their inhibiting and release/action,which were quite effective for hypothermal.