原卟啉二钠体外对丙型肝炎病毒复制子的抑制作用

Effect of Protoporphyrin Disodium in Vitro on The Anti-HCV Replicon

目的:探讨原卟啉二钠(PPN)体外对丙型肝炎病毒(HCV)的抑制作用。方法:用不同浓度PPN和利巴韦林处理HCV复制子细胞模型,MTT法检测药物的细胞毒性作用;荧光定量PCR检测HCV RNA。结果:PPN和利巴韦林浓度在1 mg·mL-1以下时,MTT法比色A值比较,实验组与对照组间差异均无显著性,无明显细胞毒性;PPN在1 mg·mL-1时对HCV RNA的抑制率为73.44%,差异有显著性(P<0.05),利巴韦林在1 mg·mL-1时对HCV RNA的抑制率为40.78%,差异无显著性,二者抑制HCV RNA的EC50分别为0.23 mg·mL-1和1.19 mg·mL-1。结论:PPN和利巴韦林浓度在1 mg·mL-1以下时对HCV Replicon细胞生长无毒性作用;PPN具有明显的体外抑制在HCV复制的作用,抑制作用强于利巴韦林。

Objective： To study the in vitro inhibitory of protoporphyrin disodium （PPN） on Hepatitis C virus. Methods：The different concentration of PPN and ribavirin water solution were respectively added into HCV Replicon cell medium. The cell toxicity of PPN was determined with MTT method. Real-time PCR was used for determining secrected HCV RNA in the culture medium. Results： PPN and ribavirin had no obvious cell toxicity at the concentration of 1 mg/ mL.When the drug concentration of PPN was 1 mg/mL, inhibitive rates of HCV RNA was 73.44%. The experimental group and control group had striking difference （ P 〈 0.05 ）, When the drug concentration of ribavirin was 1 mg/mL, inhibitive rates of HCV RNA was 40.78 %. EC50 were 0.23 mg·mL^-1 and 1.19 mg·mL^-1 respectively. Conclusion： PPN can inhibit duplication of Hepatitis C Virus in Vitro and its effect was better than ribavirin.