矮牵牛茎段植株再生体系的建立

Establishment of A Regeneration System from the Stem of Petunia hybrida Vilm

将矮牵牛Petunia hybrida Vilm的茎段接种在MS附加不同激素的培养基上进行愈伤组织诱导．结果表明：在MS＋BA1．0mol·L^-1＋NAA1．0mol·L^-1的培养基上可以形成愈伤组织，而且经过继代培养仍可形成愈伤组织；茎段或愈伤组织接种在MS＋BA1．0mol·L^-1＋NAA0．1mol·L^-1的培养基上可以形成不定芽，且诱导率达100％；健壮的高1．5～2．0cm的不定芽接种在1／2MS＋IBA0．5mol·L＋AC1．0g·L^-1的培养基上，其生长根状况良好，生根率达100％．

The stem of Petunia hybrida Vilm was cultured on MS supplemented with different combinations of hormones. Results show that MS supplemented with BA 1.0 mg. L^-1 and NAA 1.0 mg . L^-1 is a beneficial medium for callus induction and the callus can be subcultured in the same medium; that adventitious buds can be induced in MS supplemented with BA 1. 0 mol . L^-1 and NAA 0. 1 mol . L^-1 from the stem or callus and the percentage of induction is 100; and that the vigorous adventitious shouts about 1.5-2. 0 cm tall can be cultured in 1/2 MS supplemented with IBA or NAA and their highest rooting （100%） can be obtained in 1/2 MS supplemented with 0.5 mol . L^-1 IBA and 1.0g.L^-1AC 0.1 mg. L^-1.