基于SSR标记的30份玉米种质遗传完整性分析

Genetic Integrity Analysis of 30 Maize Accessions by SSR

利用70对SSR引物,对30份玉米地方品种种质进行遗传完整性检测,每一份种质包含来自两个不同繁种年份的供试亚群体。取样方法为DNA混合取样。结果表明,30份种质的供试亚群体之间的遗传相似系数除红包谷(统一编号00230080)为0.77外,其他都大于0.80。聚类分析表明,除二黄(统一编号00210055)和红包谷两份种质的亚群体出现分支外,其他28份种质亚群体分支都按照同一种质群分别聚类。本文同时探讨了个别种质亚群体之间遗传分化的原因,以及SSR标记作为玉米种质遗传完整性检测方法的可行性。

The genetic integrity of 30 accessions of maize （Zea mays L. ） stored in national genebank of China was investigated by 70 SSR primers. Each accession was represented by two sub-populations regenerated in different year. The genetic similarity between the two sub-populations of the same accession is all above 0.80, except for 0.77 in Hongbaogu （accession number 00230080）. Cluster analysis based on the similarity coefficient showed that Er-Huang（accession number 00210055） and HongBaogu diverged from each other, falling into two branches, and that each one cluster was created by two sub-populations of the same accessions, which were multiplied in different years. The reasons for the cluster divergence and feasibility of SSR for genetic integrity analysis in maize were discussed.