38份瓠瓜种质资源遗传多样性的ISSR分析

Genetic Diversity Analysis of 38 Bottle Gourd [Lagenaria siceraria(Mol.) Stand.] Accessions by ISSR

采用ISSR分子标记技术对源自中国7个省份的38份瓠瓜种质进行遗传多样性分析。12个ISSR引物共扩增出96条多态性带,平均每个引物扩增的多态性带数为8条,多态性比率平均为83.5%。聚类分析将供试的38份种质分为4个类群8组,主坐标分析将其分为4个类群10组。ISSR分子标记的分类结果与瓠瓜的农艺性状分类和地理分布有一定的相关性。

Inter-simple sequence repeat （ISSR） were used to detect the genetic diversity among 38 bottle gourd accessions from 7 provinces in China. Twelve ISSR primers produced 96 polymorphic bands, with av6raged 8 polymorphic bands each primer pair. The average percentage of polymorphic bands was 83.5%. 38 accessions were clustered into 4 groups and 8 subgroups based on the ISSR data by the method of clustering analysis, and 4 groups and 10 subgroups by the method of principal coordinates analysis. The results from ISSR molecular markers showed obvious correlation with the agronomic characteristics classification and the geographical distribution of the bottle gourd accessions.