不同细胞质埃塞俄比亚芥的获得及其生物学特性比较研究

Obtaining of Ethiopia Mustard with Different Cytoplasm by Backcrossing and Comparison of Their Characters

以埃塞俄比亚芥(Brassica carinata)为轮回亲本,分别对甘蓝型油菜(Brassica napus)和芥菜型油菜(Brassica juncea)连续回交7代,获得了具有核基因组基本相同而细胞质不同的新材料:N/BBCC和J/BBCC。两个新材料及其轮回亲本C/BBCC在生长发育时期、植株外部形态特征、POD酶活性等方面表现基本一致,但在雄性育性、脯氨酸含量及相对电导率上存在着明显差异,其中N/BBCC雄蕊发育不完善、花药瘦小、药内花粉量很少且多为无生活力的畸形花粉,表现严重败育;J/BBCC和N/BBCC的脯胺酸含量及相对电导率明显低于其轮回亲本C/BBCC。

Two new materials （N/BBCC and J/BBCC） with the similar nucleus genome from Ethiopia mustard （Brassica carinata） and different cytoplasm from Brassica napus and Brassica juncca respectively were obtained by backcrossing the B. rtapus and B. jurtcea seven generations with Ethiopia mustards as the transmigration parent. The new materials （N/BBCC and J/BBCC） and their transmigration parent （C/BBCC） put up the similar phenotypes in development stage, morphological characters, POD enzyme activity, and so on. But differences in male fertility, proline content and relative conductance rate were existed among them, in which the development of androecium in N/BBCC was abnormal, showing serious male sterility, and the proline content and relative conductance rate in N/BBCC and J/BBCC were much lower than their transmigration parent, C/BBCC.