摘要
目的探讨细胞毒性T淋巴细胞抗原4(CTLA-4)基因外显子1的49位点A/G多态性与吉兰-巴林综合征(GBS)易患率的相关性。方法采用多聚酶链反应限制性片段长度多态性(PCR-RFLP)技术分析10例吉兰-巴林综合征患者及20例正常人CTLA-4基因外显子1的49位点A/G多态性。结果CTLA-4基因的3种基因型在GBS患者组中分布情况为:AA型30.0%;AG型50.0%;GG型20.0%。在对照组中分布情况为:AA型35.0%;AG型50.0%;GG型15.0%(P>0.05)。CTLA-4/G49等位基因频率在GBS患者组为45.0%,在对照组为40%(P>0.05)。结论CTLA-4基因外显子1的49位点A/G多态性与GBS无显著性关联。
Objective To investigate the association of cytotoxic Tlymphocyte associated antigen 4（CTLA - 4） gene A/G polymorphism with susceptibility to GuiUain- Bane syndrome.Methods The A/G transition polymorphism at position 49 exonl （codonl7） of the CTLA - 4 gene was determined by polymerase chain reaction restriction fragment length polymorphism （PCR - RFLP） method in 10 patients with Guillain- Bane syndrome as well as 20 control subjects. Results The genotype （AA,AG and GG） distribution of CTLA - 4 gene A/G polymorphism was 30.0% ,50.0% and 20.0% in patients with GuiUain-Barre syndrome,and 35.0% ,50.0% and 15.0% in controls （ P 〉 0.05）.The allele frenquency of CTLA - 4/G49 in patients with GBS was 45.0% in patients with Guillain - Barre syndrome and 40.0% in controls （ P 〉 0.05）. Conclusion The polymorphism of CTLA- 4 gene was not significantly associated with Guillain - Barre syndrome.
出处
《黑龙江医学》
2007年第12期886-888,共3页
Heilongjiang Medical Journal
