摘要
目的研究抗氧化剂和吲哚生物碱在2种长春花(粉红色和白色)植株不同部位的分布。方法通过检测无酶活性的抗氧化分子含量和抗氧化酶活性测定抗氧化能力。无酶活性的抗氧化分子包括抗坏血酸、α-生育酚和还原型谷胱甘肽。抗氧化酶包括超氧化物歧化酶、抗坏血酸过氧化物酶、过氧化氢酶、过氧化物酶和多酚氧化酶。所用样品为从田间收集到的植株的根、茎、叶、花和豆荚。结果与其他部位比较,根和茎中抗氧化剂的含量较高,花和豆荚中含量最低。粉红色长春花中抗氧化剂和生物碱含量较高。叶中抗氧化酶的活性较高,但吲哚生物碱的含量在粉红色长春花的根中较高。结论对于抗氧化剂和生物碱含量而言,粉红色长春花更适合培养。
AIM To evaluate the antioxidant potential and indole alkaloid variations in different parts of two varieties (rosea and alba ) of Catharanthus roseus. METHODS The antioxidant potentials were examined in terms of non-enzymatic antioxidant molecules and activities of antioxidant enzymes. The non-enzymatic antioxidant molecules studied were ascorbic acid, ct-tocopherol and reduced glutathione. The estimated antiox- idant enzymes were superoxide dismutase, ascorbate peroxidase, catalase, peroxidase and polyphenol oxidase. The analyses were carried out in the field-collected samples. The antioxidant and alkaloid profiles were estimated from roots, stems, leaves, flowers and pods. RESULTS The roots and sterns had higher antioxidant content when compared with other parts of the plant. The lowest content was found in flowers and pods. The rosea variety was good performer in terms of antioxidant and alkaloid contents. The activities of antioxidant enzymes were higher in leaves when compared with other parts. But indole alkaloid content was found to be higher in the roots of rosea variety. CONCLUSION The pink-flowered rosea variety of Catharanthus roseus is more suitable for cultivation in terms of antioxidant and alkaloid contents.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2007年第6期487-494,共8页
Chinese Journal of Pharmacology and Toxicology
关键词
长春花
医学
草药
抗氧化剂
吲哚生物
碱类
Catharanthus roseus
medicine, herbal
antioxidants
indole alkaloids