高灵敏反相流动注射化学发光法测定抗坏血酸

Highly Sensitive Chemiluminescence System for Determination of Ascorbic Acid

基于抗坏血酸对Ｆｅ２＋－Ｌｕｍｉｎｏｌ－Ｏ２体系化学发光的抑制效应，利用反相流动注射技术，建立了一个测定抗坏血酸的高灵敏化学发光新方法。该法检出限达２×１０－１０ｇ／ｍＬ；抗坏血酸浓度在 １×１０－９～１× １０－６ｇ／ｍＬ范围内与体系化学发光强度减小量呈良好线性相关；相对标准偏差为１．９％（Ｃ＝５×１０－８ｇ／ｍＬ，ｎ＝１１）。

A highly sensitive chemiluminescence(CL) system for the determination of ascor- bic acid with reversed flow injection analysis is presented in this paper. It is based on the in- hibition of ascorbic acid in the CL reaction of luminol-Fe2+-O2, system. The linear range of calibration curve is 1 ×10-9-1 ×10-6g/mL and the detection limit is 2 ×l0-10 g/mL ascorbic acid. The system has been applied to the assay.of ascorbic acid in medicinal tablets and veg- etables with satisfactory results.