灵芝孢子油对H_(22)荷瘤小鼠肿瘤生长及瘤细胞VEGF表达的影响

LZBZY's regulatory effect on the growth of H_(22) tumor and the expression of VEGF in transplanted H_(22) tumor of mice

目的观察灵芝孢子油对荷H22瘤小鼠肿瘤生长及瘤细胞中血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,探讨该药抑制肿瘤的作用是否与抑制肿瘤血管生成有关。方法小鼠右腋皮下接种H22瘤细胞(浓度1×107/ml),0.2 ml/只,给药12天后,处死动物,剥离瘤块称重,计算肿瘤生长抑制率,用免疫组织化学法检测各组小鼠肿瘤组织中VEGF的表达程度。结果与模型组比较,灵芝孢子油对H22荷瘤小鼠肿瘤生长有明显抑制作用(P<0.05,P<0.01)。造模后各组小鼠VEGF均为阳性表达,灵芝孢子油各组可降低小鼠H22瘤组织中VEGF的阳性表达(P<0.05,P<0.01)。结论灵芝孢子油对H22移植性癌细胞生长有抑制作用,可降低瘤细胞中血管生成调控基因VEGF的表达。

Aim To observe the regulatory effect of LZBZY on the growth of H22 tumor and the expression of vascular endothelial growth factor （VEGF） in transplanted H22 tumor of mice, and explore whether the inhibition of tumor is related to the inhibition of vascular growth. Methods The mice were vaccinated with H22cells suspension （ 1 × 10^7/ml） 0.2 ml each one. The observation was made on the weight of the transplanted tumor of the mice, then the inhibitory rate was calculated. The expression of VEGF was determined by the immunohistochemical method. Results The LZBZY group had significant effect on inhibiting the tumor growth （ P 〈 0. 05, P 〈 0.01 ）, when compared with the model group ,the pathological detective results showed that the positive rate of expression of VEGF was 100% in each group with the degree difference. Moreover,the expression of VEGF was decreased obviously in LZBZY group （P 〈 0. 05,P 〈 0. 01 ）. Conclusion The LZBZY can inhibit the growth of the transplanted H22 tumor of mice and decrease the expression of the VEGF.