期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

γ-生育三烯酚对人结肠癌HT-29细胞中DNA损伤作用的研究 被引量：6

Study on γ-Tocotrienol induced DNA damage in human colon carcinoma HT-29 cells
下载PDF
导出
摘要 目的探讨γ-生育三烯酚对结肠癌细胞中DNA的损伤作用。方法γ-生育三烯酚与结肠癌HT-29细胞共培养,采用单细胞电泳法检测γ-生育三烯酚对HT-29细胞中DNA的损伤程度。结果γ-生育三烯酚对人结肠癌细胞株HT-29细胞中DNA分子有较大程度的损伤,且与处理的浓度存在一定的相关性。结论γ-生育三烯酚对结肠癌细胞具有很强的细胞毒作用,在γ-生育三烯酚作用下HT-29细胞的DNA断裂损伤增加,可能就是γ-生育三烯酚抗肿瘤效应的重要作用机制之一。 Objective To study the effect of γ-Tocotrienol on the DNA damage of colon cancer HT-29 cells. Methods The colon cancer HT-29 cells were cultured with γ-Tocotrienol in vitro. The DNA damage of colon cancer cells was determined by the single cell gel electrophoresis assay. Results γ-Tocotrienol could induce the DNA damage of colon cancer cell-lines HT-29 and show dose-response relationship. Conclusions The γ-Tocotrienol has great cytotoxic effect on colon cancer cells, the results indicating that the increasing of DNA damage in colon cancer may be a very important mechanism of γ-Tocotrienol anti-tumor effects.
作者 徐伟丽 陈炳卿 王琪 刘慧坤 孙向荣 孙文广
机构地区 哈尔滨医科大学公共卫生学院营养与食品卫生教研室
出处 《疾病控制杂志》 CAS 2007年第6期603-606,共4页 Chinese Journal of Disease Control and Prevention
基金 国家自然科学基金(30471444)
关键词 生育三烯酚类 结肠肿瘤 DNA损伤 γ-tocotrienol Colon cancer DNA damage
分类号 R735.35 [医药卫生—肿瘤]
  • 相关文献

参考文献8

  • 1Packer L, Weber SU, Rimbaeh G. Molecular aspects of alpha-tocotrienol antioxidant action and cell signalling [J]. J Nutr, 2001, 131(2) :369S-373S.
  • 2Mo H, Elson CE. Apoptosis and cell-cycle arrest in human and murine tumor cells are initiated by isoprenoids [J]. J Nutr, 1999,129(4) :804-813.
  • 3Sakai M, Okabe M, Tachibana H, et al. Apoptosis induction by gamma-tocotrienol in human hepatoma Hep3B cells [J]. J Nutr Biochem, 2006,17 (10) : 672-676.
  • 4Shah SJ, Sylvester PW. Tocotrienol-induced cytotoxicity is unrelated to mitochondrial stress apoptotic signaling in neoplastic mammary epithelial cells [J]. Biochem Cell Biol, 2005,83( 1 ) :86-95.
  • 5Sylvester PW, Shah SJ, Samant GV. Intracellular signaling mechanisms mediating the antiproliferative and apoptotic effects of gamma-tocotrienol in neoplastic mammary epithelial cells [J]. J Plant Physiol, 2005,162(7) :803-810.
  • 6Wada S, Satomi Y, Murakoshi M, et al. Tumor suppressive effects of tocotrienol in vivo and in vitro [J]. Cancer Lett, 2005,229(2):181-191.
  • 7王小红,江洪,夏明珠,杨益寿.单细胞凝胶电泳技术的研究进展及其应用[J].国外医学（临床生物化学与检验学分册）,2001,22(1):5-5. 被引量：14
  • 8Silva J, Freitas TR, Marinho JR, et al. An alkaline single-cell gel electrophoresis (comet) assay for environmental biomonitoring with native rodents [J]. Genetics and Molecular Biology, 2000, 23(1) :241-245.

二级参考文献10

  • 1Singh NP,Tice RR,Stephens RE,et al.A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides[].Mutation Research.1991
  • 2Kleinsasser NH,Wallner BC,Kastenbauer ER,et al.Comparing the genotoxic sensitivities of human peripheral blood lymphocytes and mucosa cells of the upper aerodigestive tract using the Comet assay[].Mutation Research.2000
  • 3Moller P,Wallin H,Dybdahl M,et al.Psoriasis patients with basal cell carcinoma have more repair-mediated DNA strand-breaks after UVC damage in lymphocytes than psoriasis patients without basal cell carcinoma[].Cancer Letters.2000
  • 4Pitargue M,Vaglenov A,Nosko M,et al.Evaluation of DNA damage by the comet assay in shoe workers exposed to toluene and other organic solvents[].Mutation Research.1999
  • 5Kasamatsu T,Kohda K,Kawazoe Y.Comparison of chemically induced DNA breakage in cellular and subcellular systems using the comet assay[].Mutation Research.1996
  • 6Chatterjee R,Haines GA,Perera DM,et al.Testicular and sperm DNA damage after treatment with fludarabine for chronic lymphocytic leukaemia[].Human Reproduction.2000
  • 7Everett SM,White KL,Schorah CJ,et al.In vivo DNA damage in gastric epithelial cells[].Mutation Research.2000
  • 8Eastham AM,Marples B,Kiltie AE,et al.Fibroblast radio sensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters[].British Journal of Cancer.1999
  • 9Tuo J,Loft S and Poulsen HE.Enhanced benzene-induced DNA damage in PMA-stimulated in vitro and in LPS-treated animals[].Free Radical Biology and Medicine.1999
  • 10Hannon-Fletcher Mp,O Kane MJ,Moles KW,et al.Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subject[].Mutation Research.2000

共引文献13

同被引文献88

引证文献6

二级引证文献129

疾病控制杂志
2007年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部