软骨多糖治愈的S180荷瘤小鼠免疫功能的初步研究

Study on Immune Function of the S180 Tumor-bearing Mice Cured by Cartilage Polysaccharide

目的:研究软骨多糖治愈的S180荷瘤小鼠的免疫功能及灭活S180细胞对小鼠免疫功能的影响,分析其免疫机制。方法:用MTT法测定软骨多糖治愈的S180腹水瘤小鼠的血清、脾细胞及胸腺细胞对S180细胞和MCF-7乳腺癌细胞的生长影响,并以灭活的S180细胞对健康小鼠免疫,观察其对小鼠免疫功能的影响。结果:软骨多糖治愈的S180荷瘤小鼠的血清、脾细胞及胸腺细胞对肿瘤生长均有一定抑制效果。其中在不含胎牛血清的RPMI1640培养基培养下,治愈小鼠10%的血清对S180细胞和MCF-7乳腺癌细胞的抑制率分别为64.09%、73.48%,而其20%血清对两种肿瘤细胞的抑制率则分别为88.70%、96.80%。结论:软骨多糖治愈的S180荷瘤小鼠对肿瘤有一定的免疫能力。

Objective： To study the immune function of the S180 tumor-bearing mice cured by cartilage polysaccharide, the effects of virucidal S180 tumor on immune function of the mice and the mechanisms. Methods： The influences of the blood serum, spleen cells and thymucytes of the S180 ascites tumor-bearing mice cured by cartilage polysaccharide on the S180 tumor and MCF-7 breast carcinoma cells were measured using the MTT method. Virucidal S180 tumor cells were injected into healthy mice and their effect on immune functions of the mice were investigated. Results： The blood serum, spleen cells and thymucytes of the S180 tumor-bearing mice cured by cartilage polysaccharide had definite inhibitory effects on tumor growth. When cultured in the RPMI 1640 culture-medium, the inhibition rates of 10% blood serum of the S180 tumor-bearing mice cured by cartilage polysaceharide reached 64.09 %, 73.48 %, respectively, while those of 20% blood serum of the mice were 88.70 % and 96.80 %, respectively. Conclusion： The S180 tumor-bearing mice cured by cartilage polysaceharide had definite immune functions on tumors.