大青叶中碳苷黄酮类化合物

Flavone C-glycosides from Folium Isatidis

目的对十字花科植物菘蓝(Isatis indigoticaFort.)干燥叶中的黄酮类化合物进行分离和鉴定。方法大青叶乙醇提取物经氯仿萃取后的水层经大孔树脂柱、硅胶柱和聚酰胺柱层析及重结晶等分离手段进行分离、纯化,并应用化学性质和质谱、波谱(1H-NMR、13C-NMR、HMBC)分析方法鉴定其化学结构。结果从大青叶中分离得到4个黄酮碳苷化合物,分别鉴定为异金雀花素(iso-scoparin,1)、异金雀花素-3″-O-吡喃葡萄糖苷(isoscoparin-3″-O-glucopyranoside,2)、异牡荆素(isovi-texin,3)和异牡荆-素3″-O-吡喃葡萄糖苷(isovitexin-3″-O-glucopyranoside,4)。结论化合物1、2和4为首次从菘蓝中分离得到。

Objective To isolate and identify the flavones in the leaves of Isatis indigotica Fort. （Folium Isatidis）. Methods The ethanol extract of Folium Isatidis crude drug was extracted with chloroform, and the aqueous layer was separated by macroporous resin, silica gel and polyamide column chromatography, respec- tively. The compounds obtained were identified by mass spectra and NMR data. Results The compounds separated were identified as isoscoparin （ 1 ）, isoscoparin-3＇-O-glucopyranoside （2）, isovitexin （3）, isovitexin-3＇-O-glucopyranoside （4）. Conclusions Among them, compound 1,2 and 4 are isolated from Isatis indig- otica Fort. for the first time.