菘蓝叶片离体培养与试管无性系的建立

Regeneration System and in Vitro Culture of Leaves from Isatis indigotica Fort.

以菘蓝幼嫩叶片为外植体,研究了不同激素及其组合对菘蓝离体叶片的分化方向及不定芽诱导效率的影响,建立了菘蓝叶片高效不定芽发生、植株再生体系。结果表明,在MS基本培养基中单纯添加2,4-D,不能诱导离体叶片发生不定芽,只诱导愈伤组织发生。如果在MS基本培养基中加入2,4-D和6-BA,也无不定芽发生,而添加NAA和6-BA,可以诱导其发生不定芽。在激素组合为NAA 1.0 mg/L+6-BA 0.5 mg/L时,不定芽的诱导率达到95%,在单纯加入NAA1.0 mg/L的MS基本培养基上,菘蓝叶片也可以发生不定芽,不定芽诱导率为93%,且不定芽生长健壮,不易老化,经过一段时间的培养可以直接生根并移栽成活。

High frequent adventitious shoot formation and plant-let regeneration system was established through in vitro culture of Isatis indigotica Fort. leaves. The results showed that leaf adventitious shoots could not be induced on basal MS medium only supplemented with 2,4--D or 2,4--D and 6--BA, but calli were induced. However, the adventitious shoots could be induced on basal MS medium with the addition of NAA and 6--BA. When the portions of hormones were NAA 1.0 mg/L＋ 6-- BA 0.5 mg/L, the induction rate of adventitious shoots attained 95 %. The adventitious shoots could also be induced on basal MS medium only supplemented with NAA 1.0 mg/L, with an induction rate of 93%. Meanwhile, the adventitious shoots induced by this kind of hormone were vigorous, not easy senescence, and could generate sturdy root and form integrated plants after a period of culture. This plants could be transplanted easily and successfully.