神经型烟碱乙酰胆碱受体α7亚单位基因多态性与精神分裂症的关联研究

Association between Neuronal Nicotinic Acetylcholine Receptor α7 Subunit Gene Polymorphism and Schizophrenia

目的:探讨神经型烟碱乙酰胆碱受体α7亚单位基因(CHRNA7)多态性与精神分裂症的关系。方法:采用聚合酶链反应及聚丙烯酰胺凝胶芯片技术检测精神分裂症患者(223例)与正常对照者(98例)CHRNA7基因3个(rs2337980、rs1909884、rs883473)单核苷酸多态性位点,并对分型结果进行测序鉴定。结果:两组rs1909884基因型及等位基因频率分布差异有统计学意义(P<0.05),对照组有较多的变异等位基因T。结论:CHRNA7基因rs1909884多态性可能与精神分裂症有关联,其等位基因T可能是精神分裂症的保护性因子。

Objective： To investigate the association between neuronal nicotinic acetylcholine receptor α7 subunit gene （CHRNAT） polymorphisms and schizophrenia. Methods： The polymorphisms（ rs2337980, rs1909884 and rs883473） of CHRNA7 gene were detected by PCR and polyacrylamide gel microarray in schizophrenia patients（ n =223）and healthy control（ n =98）. The results of genotyping were redetected by DNA sequencing. Results： There was significant association in either genotype or allele frequency between two groups in rs1909884（ P 〈 0.05）, more variant allele T existed in the control group. Conclusion： CHRNA7 polymorphism rs1909884 may be associated with schizophrenia, the allele T would be a protective factor.