摘要
目的:研究导流杂交基因芯片技术(HybriMax)检测高危型人乳头瘤病毒(HPV)在宫颈癌筛查上的应用价值,评估其灵敏度及特异性。方法:取150例妇科住院及门诊病人标本,采用导流杂交基因芯片技术检测高危型HPV-DNA,同时作宫颈TCT细胞学检查及行阴道镜下活体组织学检查。结果:导流杂交基因芯片技术检测高危型HPV-DNA检测检出阳性标本49例,阳性率32.6%,子宫颈癌和癌前病变筛查(HybriMax)的灵敏度为93.7%,特异性81.6%,细胞法筛查灵敏度为81.3%,特异性91.3%。结论:导流杂交基因芯片技术HPV-DNA检查可作为常规方法用于子宫颈癌的筛查。
Objective: To explore application of the detection of high - risk human papilloma virus by the technology flow - through hybridization gene coms chip in cervical cancer filtrate and to evaluate the sensitivity and the specificity of this method. Methods: 150 women patient were selected in hospital and outpatients by the technology flow -through hybridization gene coms chip for cervical cancer filtrate. As a comparison, 150 cervical TCT smears and cone biopsies from the same patients were collected to conduct cytology examination and histological detection. Results: The positive patients were 49 case accounting for 32. 6%. The sensitivity and the specificity of high - risk HPV DNA detection by the technology flow - through hybridization gene coms chip for cervical cancer were 93.7% and 81.6% , otherwise the sensitivity. And the specificity of cytology detection were 81.3% and 91.3%. Conclusion: The technology flow - through hybridization gene coms chip to detect high - risk types HPV - DNA is a useful method in cervical cancer filtrate.
出处
《中国妇幼保健》
CAS
北大核心
2007年第36期5215-5216,共2页
Maternal and Child Health Care of China
关键词
子宫颈癌
导流杂交基因芯片
人类乳头瘤病毒
Cervical cancer
Technology flow- through hybridization gene coms chip
Human papillomavirus