九牛造中总鞣质提取方法和没食子酸含量测定研究

Studies on the Methods of Extract and Determination of Total Tannins and Gallic Acids of the Root of Euphorbia hylonoma

目的:测定九牛造根中总鞣质和没食子酸含量。方法:以没食子酸为对照品,分别用紫外分光光度法及高效液相色谱法测定九牛造根中总鞣质和没食子酸含量。结果:九牛造根不同提取方法所得样品中总鞣质含量分别是水超声提取为4.375%;70%乙醇超声提取为7.24%;丙酮超声提取为3.958%;水回流提取为3.773%;70%乙醇回流提取为2.503%;丙酮回流提取为1.59%。九牛造根中没食子酸含量为0.047%。结论:采用70%乙醇超声提取所得样品中总鞣质含量最高,该方法可用于九牛造根中总鞣质的提取方法。

Objective： To determine total tannins and gallic acid of the root of Euphorbia hylonoma.Methods： With the gallic acid as reference,tannins and gallic acid of the root of Euphorbia hylonoma were determined by ultraviolet spectrophtometer and high performance liquid chromatography,respectively.Results： The content of tannins of the root of Euphorbia hylonoma were determined by different extract methods,including extracted by water,70% alcohol,acetone on supersound,the determination data were 4.375%,7.240%, 3.958%,respectively.When used recirculation method by water,70% alcohol,acetone,the determination data were the determination data are 3.773%,2.503%,1.59%,respectively.the content of gallic acid of the rootwas 0.047%.Conclusion： Content of total tannins by alcohol super sound is the highest comparing with other extract methods,this method can used in extract of total Tannins of the root of Euphorbia hylonoma.