摘要
以琯溪蜜柚田间成年树茎段为材料,研究外植体消毒方法和茎段不同成熟度等因素对无菌系建立的影响。试验结果表明,经70%酒精处理30 s、400 mg.L-1头孢拉定浸泡30 min的预处理后,用0.1%升汞溶液消毒5 min结合10%NaClO消毒30 min的方法,半木质化茎段的成功率最高,达83.3%;外植体切成5 cm长进行消毒以及培养基中添加活性炭有助于减轻外植体的褐变。
The effect of disinfection methods and developing stages of mature stem segments on the establishment of aseptic strain of Guanximiyou was studied. The results showed that after pre-treatment with 70% ethanol for 30s and 400mg·L^-1 cefotaxine for 30 min, the success rate of semi-hardened stem segments was highest, reaching 83.3 %, in the treatment of 0.1% HgC12 for 5 min and followed by the treatment of 10% NaCIO for 30 min. Cutting explants into segments of 5 cm long when disinfecting and adding activated carbon to the medium could reduce the browning rates of explants.
出处
《亚热带农业研究》
2007年第4期281-283,共3页
Subtropical Agriculture Research
基金
福建省自然科学基金项目(B0510018)
福建省教育厅科技项目(JA03062)
教育部留学回国人员科研启动基金
关键词
琯溪蜜柚
成年茎段
无菌系建立
Ctrus grandis Osbeck cv. Guanximiyou
mature stem segment
establishment of aseptic strain