桃仁蛋白对树突细胞抗原递呈功能的影响

Effect of Peach Seeds Protein on Antigen Presentation Function of Dendritic Cells

目的:探讨桃仁蛋白(psp)对树突细胞抗原递呈功能的影响。方法:制备荷S180小鼠模型,psp组给以腹腔注射桃仁蛋白,并设空白对照组;分离小鼠骨髓细胞,经IL-4、GM-CSF诱导其DC成熟,并以冻融S180抗原预致敏;在含IL-2培养体系中加入小鼠脾细胞,观察负载S180抗原T细胞对S180杀伤作用。结果:psp组对S180杀伤作用明显优于空白对照组(效靶比为40∶1时,0.638±0.044vs0.743±0.060;效靶比为20∶1时,0.561±0.041vs0.668±0.074;效靶比为10∶1时,0.461±0.036vs0.586±0.054)。结论:桃仁蛋白能够增强小鼠DC抗原递呈功能。

Objective： To explore the effection of antigen presentation function of dendritic cells （DCs） induced by peach seeds protein （psp） in vitro. Methods： Group psp and control group were injected respectively with psp and saline after they were loaded with S180 cells. Mice bone marrow cells were separated and then induced with interleukin 4（ IL-4） ,colony- stimulating factor（ GM - CSF） for maturation. The autologous S180 lysate was used to sensitize DCs. To investigate the cytotoxicity of T cells loaded S180 lysate after induced with IL -2. Results： The cytotoxicity of T cells in group psp was much higher than that of in control group（the cytotoxicity of T ceils in control group and group psp was 0.638± 0. 044 vs 0.743± 0.060, as the ratio of T ceils to S180 cells was 40：1 ;0. 561± 0. 041 vs 0. 668 ± 0. 074, as 20：1 ;0. 461 ± 0. 036 vs 0. 586 ± 0. 054, as 10：1 ）. Conclusion： psp can enhance the antigen presentation function of DCs.