急性肺损伤大鼠肺纤维增生早期检测

Pulmonary fibroproliferation detected early in the acute lung injury rats

目的探讨油酸（OA）所致大鼠急性肺损伤（ALI）早期肺纤维组织增生情况及其可能的纤维化机制。方法应用尾静脉注射OA复制大鼠ALI模型。48只健康SD大鼠（体质量0．29～0．31kg，雌雄不拘）随机分为4组：生理盐水对照组（NS组，n=12），OA致伤1d组（OA1组，n=12），OA致伤3d组（OA2组，n=12），OA致伤7d组（0A3组，n=12）。OA致伤组以0．12ml／kg的OA尾静脉注射，NS组注射同等量的生理盐水。观察肺部病理变化，检测肺湿／干重比（W／D值），测定动脉血氧分压（PaO2）、动脉血二氧化碳分压（PaCO2）、pH值及血浆Ⅲ型前胶原肽N末端（PⅢNP）浓度，测支气管肺泡灌洗液（BALF）中总蛋白（TP）、总磷脂（TPL）、磷脂酰胆碱（PC）和PⅢNP浓度。结果①与NS组比较，OA组肺组织可见明显充血、水肿、出血等ALI表现，各时间点BALF中TPL、PC／TPL明显减少（P〈0．01），而TP、W／D值、PⅢNP及血浆PⅢNP显著升高（P〈0．01），动脉血PaO2、PaCO2、pH值也有明显变化（P〈0．01）。②OA组各时间点血浆与BALF中PmNP浓度呈正相关（rOA1=0．864，P〈0．01；rOA2=0．829，P〈0．01；rOA3=0．874，P〈0．01）。结论AU早期肺就发生纤维组织增生；ALI时PⅢNP浓度显著升高，通过检测BALF和血浆中的PⅢNP浓度可能有助于早期诊治ALI.

Objective To investigate pulmonary fibroproliferation early in the acute lung injury （ALI） rats by injecting oleie acid（OA） into tail vein and the mechanism of pulmonary fibroproliferation. Methods The model of ALI in rats was reproduced by injecting OA into the tail vein. Forty-eight adult Sprague- Dawley rats（mass 290-310 g, regardless of sex） were randomly divided into four groups： normal control group treated by injecting saline （NS group, n = 12） ;ALI group one induced by injecting oleie acid （0.12 ml/kg ） at 1 day（OA group 1, n =12） ;ALI group two induced by injecting oleie acid at 3 day （OA group 2, n = 12） ;ALI group three induced by injecting oleie acid at 7 day （OA group 3, n = 12）. Arterial partial pressure of oxygen （PaO2）, arterial partial pressure of carbon dioxide （PaCO2）, power of hydrogen （pH） and plasma PⅢNP concentration level were evaluated. Investigating histopathology and calculating wet/dry mass ratio（W/D）, and determining the total proteins （TP）, total phospholipids （TPL）, phosphatidyleholine （PC） and P Ⅲ NP concentration in the bronehoalveolar lavage fluid （BALF）. Results （1） In OA group, compared with NS group, there showed obvious pathological manifestations of ALI, such as hyperemia, edema,hemorrhage. TPL and PC/TPL in the BALF were significantly and progressively decreased （ P 〈 0.01）, whereas TP, W/D and P Ⅲ NP concentration were progressively increased （ P 〈0.01 ）. There were significant differences in PaO2 ,PaCO2 ,pH between NS group and OA group （ P 〈0.01）. （2）The Pm NP concentrations in the BALF were positively correlated to those in the plasma in OA at the same time point （rOA1 = 0. 864, P G0.01; rOA2 =0.829, P 〈0.01; rOA3 =0.874, P 〈0.01）. Conclusions Pulmonary fibroproliferation occurs at the early phase of ALL ALI may be diagnosed and treated at the early phase by detecting the Pm NP concentrations in BALF and plasma.