关苍术乙酸乙酯提取物对大鼠缺血再灌注损伤心肌保护作用的研究

The Protective Effect of Ethyl Acetate Extract from Japanese Artactylodes Rhizome on Myocardial Ischemical Reperfusion Injury in Rats

目的:研究关苍术乙酸乙酯提取物对大鼠心肌缺血再灌注损伤的保护作用及可能机制。方法:将50只大鼠随机分为假手术组、模型组、关苍术乙酸乙酯提取物低、高剂量(200、400mg.kg-1)组及维拉帕米(阳性药物)组,各组心肌缺血40min。分别于再灌注30min后取心肌组织,测定谷胱甘肽过氧化物酶(GSH-Px)的活力,心肌线粒体钙(Mit-Ca2+)的含量,心肌细胞膜Na+-K+-ATP酶、Mg2+-ATP酶的活力。结果:再灌注30min后,关苍术乙酸乙酯提取物组心肌组织中的GSH-Px活力显著高于模型组;心肌Mit-Ca2+的含量显著低于模型组;心肌细胞膜Na+-K+-ATP酶、Mg2+-ATP酶的活力显著高于模型组。结论:关苍术乙酸乙酯提取物通过增加心肌组织中GSH-Px酶的活力,降低Mit-Ca2+的含量,增加心肌细胞膜Na+-K+-ATP酶、Mg2+-ATP酶的活力,来发挥对大鼠心肌缺血再灌注损伤的保护作用。

OBJECTIVE： To study the protective effect of ethyl acetate extract from Japanese Artactylodes Rhizome on myocardial ischemical reperfusion injury in rats and its possible mechanism, METHODS： 50 Wistar rats were divided randomly into 5 groups： sham group, model group, medicine （ethyl acetate extract from Japanese Artactylodes Rhizome at dosage of 200mg · kg ^-1 and 400mg · kg^-1, respectively） group and verapamil（positive medicine） group, Rats in medicine group, model group and positive group were subjected to myocardial ischemia for 40min followed by reperfusion for 30 min, then muscular tissues were sampled for the determination of the activity of GSH- Px, the content of mitochondrial calcium （Mit- Ca^2 ＋）,and the activities of Na^ ＋-K ^ ＋-ATPase and Mg^2 ＋- ATPase in myocardium. RESULTS： As compared with model group, in the group treated with ethyl acetate extract from Japanese Artactylodes Rhizome 30min after reperfusion, the activity of GSH-Px was significantly higher, the content of myocardial mitochondrial calcium was significantly lower, and the activities of Na^ ＋ - K ^＋-ATPase, Mg^2＋- ATPase were significantly higher.CONCLUSION： The protective effect of ethyl acetate extract from Japanese Artactylodes Rhizome on myocardial ischemical reperfusion injury might be achieved through enhancing the activity of myocardial GSH - Px, lowering the content of myocardial mitochondrial calcium and enhancing the activities of Na ^＋- K ^＋ - ATPase and Mg^2 ＋ - ATPase of cellular membranes.