HPLC法测定臁疮丸中丹参酮Ⅱ_A的含量

Assaying of Tanshinone Ⅱ_A in Lianchuang Pills by HPLC

目的：建立以高效液相色谱法测定臁疮丸中丹参酮ⅡA含量的方法。方法：色谱柱为Agilent Zorbax SB—C18（250mm×4．6mm,5μm），流动相为甲醇-水（75：25），流速为1．0mL·min^-1柱温为25℃，检测波长为270nm。结果：丹参酮ⅡA进样量在0．0202-0．2020μg范围内与峰面积积分值呈良好的线性关系（r=0．9999）；平均加样回收率为99．79％，RSD=1．01％（n=6）。结论：本方法快捷、简便，结果准确，可用于该制剂的质量控制。

OBJECTIVE： To develop an HPLC method for the determination of Tanshinone ⅡA in Lianchuang pills. METHODS： The chromatographic separation was performed on Agilent Zorbax SB- C18 （250mm × 4.6mm,5μm） column with column temperature at 25℃ . The mobile phased consisted of methanol water （75 ： 25） at a flow rate of 1.0mL · min^-1.The detection wavelength was set at 270nm.RESULTS：The linear range of Tanshinone Ⅱ A was 0. 020 2-0.202 0μg（ r = 0.999 9）. The average recovery was 99.79% （RSD = 1.01%, n = 6）. CONCLUSION： The method is simple, rapid and accurate, and it could be used for the quality control of Lianchuang pills.